| Title |
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Microbial biosurfactants for potential use in oil-spill remediation |
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| Session Title |
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Technological, Environmental and Policy Developments for Improved Research and Operations in the Gulf of Mexico |
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| Presenter Last Name |
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Nyman |
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| Presenter First Name |
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Andrew |
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| Presenter Affiliation |
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LSU AgCenter |
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| Presenter Email |
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lamsal@iastate.edu |
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| Authors |
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Andy Nyman, LSU AgCenter; B.P. Lamsal, Iowa State University; M.E. Marti, Iowa State University; W.J. Colonna, Iowa State University; P. Patra, Columbia University; C. Green, LSU AgCenter; A.J. Kuhl, LSU AgCenter; K. Jarrell, Modular Genetics, Inc.; P. Somasundaran, Columbia University; C.E. Glatz, Iowa State University |
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| Abstract |
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Biosurfactants are biodegradable, and less toxic and have potential for oil-spill remediation. Lipopeptide Surfactin from Bacillus subtilis, and engineered variant, fatty acyl-glutamate (FA-Glu) were produced via fermentation. Isoforms of these biosurfactants will be purified and surface-active properties, and toxicity studied. Glucose-based media had surfactin 15X higher than FA-Glu in foam-enriched fraction. Critical micelle concentration (CMC), and dispersant-to-oil ratio (DOR), indicated that biosurfactants were comparable to conventional surfactants in DI water;at 0.35 ppm saline, CMCs were higher. DOR of surfactin was 1:96 in DI-water and 1:12 in 0.35 ppm NaCl. DOR of FA-Glu increased from 1:1.15 (DI-water) to 1: 3.5 (0.35 ppm NaCl). Surfactin was 10X toxic than FA-Glu to 7-8 d old Gulf killifish, compared to intermediate toxicity for sodium laurel sulfate. The basic structure-function relationship for these biosurfactants is being investigated so as to design biochemicals for possible oil-spill remediation, with minimal or no adverse environmental effect(s). |
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| Presentation Type |
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Oral Presentation |
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| Session Number |
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017 |
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| Date |
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Monday, January 21 |
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| Time |
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1215 |
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| Location |
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Acadia |
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| Student? |
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No |
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| Invited Talk? |
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No |
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